KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Guinea pig polyclonal anti-insulin | Agilent | Cat# A0564; RRID: AB_10013624 |
| Rabbit polyclonal anti-NPC1 | Abcam | Cat# ab134113; RRID: AB_2734695 |
| Rat anti-LAMP2 antibody [GL2A7] | Abcam | Cat# ab13524; RRID: AB_213473 |
| Rabbit polyclonal anti-GM 130 | Sigma-Aldrich | Cat# G7295; RRID: AB_532244 |
| Mouse monoclonal anti-LAMP2 | BD Biosciences | Cat# 555803; RRID: AB_39613 |
| Mouse monoclonal APC-conjugated HIC1 | Novus Biologicals | NBP1–18946APC |
| Mouse monoclonal anti-TM4SF4 AF488-conjugated | R&D Systems | FAB7998G |
| Mouse monoclonal anti-Glucagon | Sigma-Aldrich | Cat# G2654; RRID: AB_259852 |
| Sheep polyclonal anti-somatostatin | American Research Products | Cat# 13–2366; RRID: AB_1542966 |
| Alexa Fluor® 488 AffiniPure Donkey Anti-Guinea Pig IgG (H+L) | Jackson Immunoresearch | Cat# 706-545-148; RRID: AB_2340472 |
| Alexa Fluor® 647 AffiniPure Donkey Anti-Mouse IgG (H+L) | Jackson Immunoresearch | Cat# 715-605-151; RRID: AB_2340863 |
| Cy3 AffiniPure Donkey Anti-Rabbit IgG (H+L) | Jackson Immunoresearch | Cat# 711-165-152; RRID: AB_2307443 |
| Cy5 AffiniPure Donkey Anti-Rat IgG (H+L) | Jackson Immunoresearch | Cat# 712-175-153; RRID: AB_2340672 |
| Cy3 AffiniPure Donkey Anti-Sheep IgG (H+L) | Jackson Immunoresearch | Cat# 713-165-147; RRID: AB_2315778 |
| Rabbit anti-ZnT8 | University of Denver, Howard Davidson Group | N/A |
| Bacterial and Virus Strains | ||
| Deidentified human FFPE pancreatic sections | nPOD | Donor# 6004 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| LysoTracker Green DND-26 | ThermoFisher Scientific | Cat# L7526 |
| 2-Morpholinoethanesulfonic Acid | TCI America | Cat# 50-014-40052 |
| ZIGIR | This paper | N/A |
| HEPES Buffer | Sigma-Aldrich | Cat# H3575 |
| N-Cyclohexyl-2-aminoethanesulfonic acid | Frontier Scientific | Cat# 50-144-8697 |
| TPEN | Sigma-Aldrich | Cat# P4413 |
| DNase I recombinant, RNase-free | Roche | Cat# 4716728001 |
| Cryogel | EMS | Cat# 62806–01 |
| Collagenase from Clostridium histolyticum | Sigma-Aldrich | Cat# C7657 |
| Normal Donkey Serum | Jackson Immuno Research Labs | Cat# NC9624464 |
| RPMI-1640 medium | GIBCO | Cat# 11875–093 |
| TSQ | Enzo Life Sciences | Cat# ENZ-52153 |
| FluoZin-3 AM | ThermoFisher Scientific | Cat# F24195 |
| Newport Green PDX acetoxymethyl ether | Invitrogen | Cat#N24191 |
| Zinpyr-4 | Strem Chemicals Inc | Cat# 07–0312 |
| 1-Hydroxypyridine-2-thione zinc salt | Sigma-Aldrich | Cat# H6377 |
| Critical Commercial Assays | ||
| HTFR Insulin High Range Assay kit | CisBio | Cat# 62IN1PEG |
| Experimental Models: Cell Lines | ||
| Mouse insulinoma beta-cells MIN6 | Miyazaki et al., 1990 | N/A |
| INS-1 832/13 Rat Insulinoma Cell Line | EMD Millipore | SCC207 |
| Human Bone Osteosarcoma Epithelial Cells (U2OS Line) | Joachim Seemann Lab | N/A |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J mice | Jackson Laboratory | Stock No:000664 |
| Software and Algorithms | ||
| ImageJ | https://imagej.nih.gov/ij/ | N/A |
| ChemDraw 19.0.22 | PerkinElmer Informatics | N/A |
| Graphpad Prism 8.0.3 | GraphPad Software | N/A |
| Other | ||
| Histosette II Tissue Cassette | Fisher Scientific | Cat# 15-182-701c |
| Superfrost Plus Slides | Fisher Scientific | Cat# 12-550-15 |
| Fluoromount-G | SouthernBiotech | Cat# 0100-01 |
| Ludin imaging chamber | Life Imaging Services | https://www.lis.ch/thechamber.html |