Fig. 1.

Development of arbuscular mycorrhizal colonization in the wild type, sunn, and rdn1 over time. Root length colonization (%) of arbuscules (A) and vesicles (D) in the wild type, sunn, and rdn1 at four different time points in days post-inoculation (dpi) based on McGonigle et al. (1990). The numbers of arbuscules (B, C) and vesicles (E, F) were counted within a pre-defined square of 0.08 mm². To extract differences in arbuscule and vesicle intensities, observations were allocated to four groups based on the number of arbuscules (0, 1–12, 13–24, >24) or vesicles (0, 1–2, 3–4, >4) counted per observation. Observations per group are presented as a percentage (%). Observations were made in 100 individual root segments in each of four biological replicates per genotype. Bars are average values ±SEs. Statistically significant differences between averages within each group are labeled with a different letter based on one-way ANOVA followed by Tukey´s post-hoc test (P<0.05).