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. 2020 Apr 20;71(16):4972–4984. doi: 10.1093/jxb/eraa193

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© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Root length colonization by mycorrhizal arbuscules and vesicles in different genetic backgrounds. (A) Composite plants overexpressing p35S::CLE53 compared with empty vector controls (p35S::DsRed) in the wild-type background. (B) Composite plants overexpressing p35S::CLE53 compared with empty vector controls (p35S::DsRed) in the wild-type, sunn, and rdn1 backgrounds. (C) Composite wild-type plants overexpressing a native version of MtCLE53 (p35S::CLE53) and a mutated version with prolines changed to glycines (p35S::CLE53_Pro4,7) compared with the empty vector control (p35S::DsRed). Root length colonization was assessed in individual root segments (n=100) in four biological replicates. Bars are averages ± SEs. Statistically significant differences between averages within each group are labeled with different letter based on one-way ANOVA followed by Tukey´s post-hoc test (P<0.05). (D) The mtcle53 mutant line NF10070 has a Tnt1 insert 214 bp upstream of the MtCLE53 ATG start codon. Based on PCR on genomic DNA (gDNA) using MtCLE53-specific forward (fwd) and reverse (rev) primers, homozygous mtcle53 mutants were identified in a segregating population of NF10070 siblings. (E) Relative expression levels of MtCLE53 in mtcle53 and heterozygous siblings based on qRT–PCR of AMF-colonized roots. Bars are averages ± SDs. Significant differences are marked with an asterisk as calculated by two-sided Student’s t-test (P<0.05). (F) Root length colonization by arbuscules and vesicles in mtcle53 and siblings in analyzed root segments (n=160) of eight biological replicates. Bars are averages ± SEs. Statistically significant differences between averages within each group are labeled with a different letter based on one-way ANOVA followed by Tukey’s post-hoc test (P<0.05)