Fig. 2.

Responses of NbPLC2s-silenced Nicotiana benthamiana plants to virulent compatible strain of Ralstonia solanacearum. Leaves of plants at 8 weeks old were infiltrated with R. solanacearum OE1-1. Plants were silenced using VIGS. (A) Bacterial populations of R. solanacearum following inoculation. Data are means (±SD) of n=5 replicates. (B) Disease development of bacterial wilt according to a disease index scale of 0–4. Data are means of n=10 plants; for clarity, the error bars are not shown. (C) Images of control and VIGS plants at 12 d after inoculation. Significant differences between control and VIGS plants were determined using Student’s t-test: *P<0.05.