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. 2020 Aug 6;16(8):e1008696. doi: 10.1371/journal.ppat.1008696

Fig 1. Diminished cytokine production by HLA-B*35Px-restricted CD8+ T cells.

Fig 1

(A) Cumulative data showing quantification of IFN-γ secreting cells (spot count)/ 106 PBMCs following stimulation with different 15 mer peptides restricted by HLA-B*27/B57, non-HLA-B*27/B57 (HLA-A*03, A*02, A*24, A*26 and B*40) and HLA-B*35Px/B*53 using ELISpot assay. (B) Cumulative data showing the breadth of IFN-γ response to different HIV-proteins in PBMCs of HLA-B*35Px individuals using ELISpot assay. (C) Representative flow cytometry plots, and (D) cumulative data showing percentages of IL-2 secreting CD8+ T cells restricted by HLA-B*27/B57, non-HLA-B*27/B57 and HLA-B*35 in HIV-infected individuals following stimulation with their cognate epitopes (2 μg/ml) for 72 hrs as measured by ICS. (E) Representative flow cytometry plots, and (F) cumulative data showing percentages of TNF-α secreting CD8+ T cells restricted by HLA-B*27/B57, non-HLA-B*27/B57 and HLA-B*35 in HIV-infected individuals following stimulation of PBMCs with their cognate epitopes (2 μg/ml) for 72 hrs using ICS. (G) Representative flow cytometry plots, and (H) cumulative data showing percentages of IFN-γ secreting CD8+ T cells restricted by HLA-B*27/B57, non-HLA-B*27/B57 and HLA-B*35 in HIV-infected individuals following stimulation with their cognate epitopes (2 μg/ml) for 72 hrs as measured by ICS. (I) Cumulative data showing quantification of IFN-γ secreting cells (spot count)/ 106 PBMCs following stimulation with different epitopes restricted by non-HLA-B*27/B57 (HLA-A*03, A*01, A*02, A*24, A*11, B*38, B*08 and B*44) and HLA-B*35Px/B*53 from 5 HLA-B35/B53 individuals using ELISpot assay. Each point represents data from an epitope. Bar, mean ± one standard error.