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. Author manuscript; available in PMC: 2020 Aug 6.
Published in final edited form as: Cell Rep. 2020 Jul 14;32(2):107899. doi: 10.1016/j.celrep.2020.107899

Figure 4. Reactivation of Negative Valence PL-to-BLA Projections Leads to a Social Deficit.

Figure 4.

(A) Diagram of experimental timeline. Cre-dependent virus was delivered to the PL of FosTRAP mice. Two weeks later, the same mice underwent a foot-shock protocol and immediately were injected with 4-hydroxy-tamoxifen (4-TM) to induce Cre-mediated expression of ChR2. Four weeks later, optical fibers were implanted targeting the BLA. One and 2 weeks afterward, social approach tests took place counterbalanced for control versus light-on conditions.

(B) Representative image of the expression of ChR2 through Cre-dependent FosTRAP method.

(C) Representative heatmap of control (top) and light-on (bottom) condition of three-chamber social approach assay.

(D) Quantification of time spent in each chamber of the three-chamber social approach assay.

Paired-samples t tests were used to compare time spent in the chambers containing a mouse in a tube and an empty tube. Data are represented as mean ± SEM. **p < 0.01.

See also Figure S4 relating to these experiments.