Skip to main content
. 2020 Jul 30;29(15):1007–1015. doi: 10.1089/scd.2020.0070

FIG. 1.

FIG. 1.

Anti-DKK1, but not anti-SOST, enhances the osteogenic differentiation of human ASCs. (A, B) Gene expression of DKK1 and SOST across time during the osteogenic differentiation of human ASC (0–10 days of differentiation). Each group of different time points was compared with that of 0 days. (C) Effects of anti-DKK1 on ASC proliferation, assessed by MTS assay at 24–72 h (1 μg/mL). (D, E) Effects of anti-DKK1 on ASC osteogenic differentiation, assessed by alizarin red staining and quantification after 7 days of differentiation (0.5–4 μg/mL). Whole well images shown. (F, G) Effects of anti-SOST on ASC osteogenic differentiation, assessed by alizarin red staining and quantification after 7 days of differentiation (0.5–5 μg/mL). All experiments performed with an appropriate isotype IgG control. Experiments performed in at least experimental and biological triplicate. Error bars represent one standard deviation. *P < 0.05; **P < 0.01; ***P < 0.001. ASC, adipose-derived stem/stromal cell; DKK1, Dickkopf 1; SOST, sclerostin.