FIG. 4.

Anti-DKK1-induced osteogenic differentiation in human ASCs is related to both basal DKK1 and SOST expression. (A) Fold change in alizarin red staining among anti-DKK1 versus IgG (1 μg/mL) within five distinct human ASC preparations. See Supplementary Table S1 for a description of patient demographics. (B) Relative baseline DKK1 expression among each ASC preparation, as determined by qPCR. (C) Relative baseline SOST expression among each ASC preparation, as determined by qPCR. Each data point represents a mean value, while error bars represent one standard deviation. qPCR, real-time polymerase chain reaction.