Table 1. Animals displaying different microsatellite alleles representing Leishmania infantum parasites infecting distinct tissues.

For DNA isolation directly from tissues, only spleen samples were employed, but DNA was also isolated from cultured parasites isolated from diverse tissues from naturally infected dogs living in an endemic area of visceral leishmaniasis in Brazil.

Animal ID	Sample type1	Sample ID	Tissue2	Marker		Scenario (depicted in Fig 3)
				Allele 1	Allele 2
107				Lm4TA
	Tissue	107	Spleen	81	83
	Strain	3130	Bone Marrow	79	83	6
110				Lm4TA
	Tissue	110	Spleen	83	83	6
	Strain	3131	Bone Marrow	81	81
113*				Lm2TG
	Tissue	113	Spleen	140	140	7
	Strain	3132	Bone Marrow	142	142
	Strain	3158	Bone Marrow	142	142
				Lm4TA
	Tissue	113	Spleen	-	-
	Strain	3132	Bone Marrow	81	81
	Strain	3158	Bone Marrow	83	83
116				Li41–56
	Strain	3133 – 1st	Bone Marrow	88	90	9
	Strain	3133 – 2nd	Bone Marrow	90	90
121				Li22–35
	Tissue	121	Spleen	94	94	8
	Strain	3134	Liver	94	94
	Strain	3135	Liver	96	96
				Lm4TA
	Tissue	121	Spleen	83	83
	Strain	3134	Liver	83	83
	Strain	3135	Liver	79	79
				Li 71–33
	Tissue	121	Spleen	105	105
	Strain	3134	Liver	105	105
	Strain	3135	Liver	106	106
				Li 71–52
	Tissue	121	Spleen	-	-
	Strain	3134	Liver	110	110
	Strain	3135	Liver	108	108
				Li71–70
	Tissue	121	Spleen	100	100
	Strain	3134	Liver	100	100
	Strain	3135	Liver	98	98
124				Lm2TG
	Tissue	124	Spleen	138	142	1
	Strain	3137	Spleen	136	142
239				Li22–35
	Tissue	239	Spleen	94	94	1
	Strain	3201	Spleen	94	96
244				Li 71–33
	Tissue	244	Spleen	105	107
	Strain	3206	Spleen	107	107	4
	Strain	3205	Bone Marrow	105	107
	Strain	3207	Ascites	107	107
				Li22–35
	Tissue	244	Spleen	94	94
	Strain	3206	Spleen	94	96
	Strain	3205	Bone Marrow	94	96
	Strain	3207	Ascites	94	96
251				Li22–35
	Strain	3210	Spleen	94	96	10
	Strain	3211	Liver	94	94
252				Lm4TA
	Tissue	252	Spleen	79	83
	Strain	3213	Spleen	83	83	3
	Strain	3212	Bone Marrow	79	83
254				Lm2TG
	Tissue	254	Spleen	142	142	1
	Strain	3215	Spleen	128	142
256*				Lm2TG
	Tissue	256	Spleen	142	142	2
	Strain	3217	Spleen	144	144
	Strain	3218	Liver	142	142
				Lm4TA
	Tissue	256	Spleen	83	83
	Strain	3217	Spleen	81	81
	Strain	3218	Liver	83	83
257				Lm2TG
	Tissue	257	Spleen	142	142
	Strain	3220	Spleen	142	144	5
	Strain	3219	Bone Marrow	142	146
	Strain	3221	Liver	142	144
291*				Lm2TG
	Tissue	291	Spleen	142	142	1
	Strain	3323	Spleen	142	144
				Lm4TA
	Tissue	291	Spleen	83	83
	Strain	3323	Spleen	85	85

¹Sample type—type of sample employed for DNA extraction; Tissue indicates that DNA was extracted directly from a spleen fragment and Strain indicates that DNA was extracted from cultured parasites.

²Tissue–the tissue from which DNA was extracted or the parasite (strain) from which DNA was isolated; *Different population assignment was made after STRUCTURE analysis