Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | C57Bl/6J (Mouse, male) |
Envigo | Stock #057 | MGI:2164189 |
| Genetic reagent (Mus musculus) | C57Bl/6NHsd (Mouse, male) |
Envigo | Stock #044 | MGI:2161078 |
| Genetic reagent (M. musculus) |
C57Bl/6J (Mouse, male) |
Charles River | Stock #632 | MGI:3028467 |
| Genetic reagent (M. musculus) |
C57Bl/6J (Mouse, male) |
Janvier labs | N/A | MGI:2670020 |
| Genetic reagent (M. musculus) |
PomceGFP
(Mouse, male) |
Jackson Laboratories | Stock #: 009593 | MGI:3851684 |
| Genetic reagent (M. musculus) |
Cckires-Cre
(Mouse, male) |
Jackson Laboratories | Stock #: 012706 | MGI:5014249 |
| Genetic reagent (M. musculus) |
Crhires-Cre
(Mouse, male) |
Jackson Laboratories | Stock #: 012704 | MGI:4452101 |
| Genetic reagent (M. musculus) |
Slc17a6ires-Cre
(Mouse, male) |
Jackson Laboratories | Stock #: 016963 | MGI:5300532 |
| Genetic reagent (M. musculus) |
Rosa26-loxSTOPlox-eYFP (Mouse, male) | Jackson Laboratories | Stock #: 006148 | MGI:3621481 |
| Genetic reagent (M. musculus) |
GcgiCre
(Mouse, male) |
Parker et al., 2012 PMID:22638549 | N/A | MGI:5432481 |
| Genetic reagent (M. musculus) |
Prlhires-Cre
(Mouse, male) |
Dodd et al., 2014 PMID:25176149 | N/A | MGI:5634277 |
| Genetic reagent (M. musculus) |
CalcaCre
(Mouse, male) |
Carter et al., 2013 PMID:24121436 | N/A | MGI:5559692 |
| Genetic reagent (Rattus norvegicus) | Sprague Dawley (Rat, male) | Envigo | Stock #: SD-002 | N/A |
| Antibody | anti-cFos (Rabbit polyclonal) | Santa Cruz | Cat.# SC52 RRID:AB_2106783 |
Primary antibody (1:500) IHC |
| Antibody | anti-DS Red (Goat polyclonal) | Santa Cruz | Cat.# 33353 RRID:AB_639924 |
Primary antibody (1:500) IHC |
| Antibody | anti-GFP (Chicken polyclonal) | Abcam | Cat.# 13970 RRID:AB_300798 |
Primary antibody (1:2000) IHC |
| Antibody | anti-GFRAL (Sheep polyclonal) |
Thermofisher | Cat.# PA5-47769 RRID:AB_2607220 |
Primary antibody (1:200) IHC |
| Antibody | anti-GLP1 (Rabbit polyclonal) | PenLabs | Cat.#. T-4363 RRID:AB_518978 |
Primary antibody (1:2000) IHC |
| Antibody | anti-PKCδ (Mouse monoclonal) | BD Biosciences | Cat.#. 610398 RRID:AB_397781 |
Primary antibody (1:500) IHC |
| Antibody | anti-TH (Rabbit polyclonal) | AbCam | Cat.# AB112 RRID:AB_297840 |
Primary antibody (1:2000) IHC |
| Antibody | anti-TH (Sheep polyclonal) | Millipore | Cat.# AB1542 RRID:AB_90755 |
Primary antibody (1:1000) IHC |
| Antibody | anti-chicken, Alexa Fluor 488 (Donkey polyclonal) | Jackson ImmunoResearch | Cat.# 703-545-155 RRID:AB_2340375 |
Secondary antibody (1:1000) IHC |
| Antibody | anti-mouse, Alexa Fluor 594 (Donkey polyclonal) | Jackson ImmunoResearch | Cat.# 715-585-150 RRID:AB_2340854 |
Secondary antibody (1:1000) IHC |
| Antibody | anti-rabbit, Alexa Fluor 350 (Donkey polyclonal) | Molecular Probes | Cat.# A10039 RRID:AB_2534015 |
Secondary antibody (1:1000) IHC |
| Antibody | anti-Sheep, Alexa Fluor 350 (Donkey polyclonal) | Molecular Probes | Cat.# A21097 RRID:AB_10376162 |
Secondary antibody (1:1000) IHC |
| Antibody | anti-sheep, Alexa Fluor 594 (Donkey polyclonal) | Molecular Probes | Cat.# A11016 RRID:AB_10562537 |
Secondary antibody (1:1000) IHC |
| Antibody | Anti-GFRAL (Mouse monoclonal) | Emmerson et al., 2017 PMID:28846098 | mIgG1 GFRAL 8A2 | Subcutaneous injection (0–10 mg/kg) |
| Recombinant DNA reagent | AAV8-hSyn-DIO-mCherry | Dr Bryan Roth Addgene |
Cat.# 50459-AAV8 | N/A |
| Recombinant DNA reagent | AAV5-flex-taCasp3-TEVp | Dr Nirao Shah University of North Carolina Vector Core |
N/A | PMID:23663785 |
| Sequence-based reagent | Gfral | Advanced Cell Diagnostics | Cat.# 417021-C3 | RNAScope mRNA probe |
| Sequence-based reagent | Cck | Advanced Cell Diagnostics | Cat.# 402271-C1 | RNAScope mRNA probe |
| Sequence-based reagent | Cckr1 | Advanced Cell Diagnostics | Cat.# 313751-C1 | RNAScope mRNA probe |
| Sequence-based reagent | Calca | Advanced Cell Diagnostics | Cat.# 420361-C2 | RNAScope mRNA probe |
| Peptide, recombinant protein | GDF15 | R and D Systems | Cat.# 9279-GD | (4 nmol/kg) |
| Peptide, recombinant protein | Streptavadin 488 | Jackson ImmunoResearch | Cat.# 016-540-084 RRID:AB_2337249 |
(1:1000) IHC |
| Peptide, recombinant protein | Streptavadin 594 | Jackson ImmunoResearch | Cat.# 016-580-084 RRID:AB_2337250 |
(1:1000) IHC |
| Commercial assay or kit | Mouse/rat GDF15 ELISA | R and D Systems | Cat.# MGD-150 | N/A |
| Commercial assay or kit | RNAscope Multiplex Fluorescent Assay | Advanced Cell Diagnostics | Cat # 323100 | |
| Chemical compound, drug | Devazepide | Tocris Bioscience | Cat.# 2304 | (1 mg/kg) |
| Chemical compound, drug | Lithium chloride (LiCl) | Sigma | Cat # L9650 (mouse) Cat.# 73036 (rat) |
Mouse (128 mg/kg) Rat (128 mg/kg) |
| Chemical compound, drug | Hydroxystibamidine (Fluoro-Gold) | Invitrogen, Thermofisher | Cat.# H22845 | 4% in H2O |
| Chemical compound, drug | Cisplatin | Sigma Aldrich | Cat.# PHR1624 | (4 mg/kg) |
| Software, algorithm | Prism | GraphPad | RRID:SCR_002798 | Version 7 |
| Software, algorithm | Fiji | ImageJ | RRID:SCR_002285 | Version 2.0.0-rc-69/1.52 p |
| Software, algorithm | Micromanager | ImageJ | RRID:SCR_016865 | Version 1.4.23 |
| Software, algorithm | Smart | Panlab, Harvard Biosciences/Biochrom Ltd | RRID:SCR_002852 | Version 3.0 |