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. 2020 Aug 6;9:e57381. doi: 10.7554/eLife.57381

Figure 2. Deletion of DYRK2 affects activation of Hh signaling in mouse development.

(A) In situ hybridization of Gli1 in the craniofacial region in wild-type and Dyrk2-/- embryos from the sagittal plane at E14.5. (B) Immunoblotting of GLI1 in extracts from the limbs of wild-type and Dyrk2-/- embryos at E13.5. GAPDH serves as a loading control. (C) qPCR of Gli1, Ptch1, and Shh in the limbs from wild-type and Dyrk2-/- embryos at E13.5. (D, E) Repression of Foxf2-expression in the craniofacial region of Dyrk2-/- mice. (D) In situ hybridization of Foxf2 in the craniofacial region in wild-type and Dyrk2-/- embryos from the sagittal plane at E14.5. (E) qPCR of Foxf2 in the mandibular arch from wild-type and Dyrk2-/- embryos at E10.5. Hypoxanthine phosphoribosyltransferase (Hprt) in (C and E) was used as an internal standard, and fold change was calculated by comparing expression levels relative to those of wild-type. Data are presented as the means ± SEM (n = 3 biological replicates). The statistical significance between wild-type and Dyrk2-/- was determined by the Student’s t-test. (*) p<0.05, (**) p<0.01. t, tongue; ul, upper lip. Scale bars, 500 µm.

Figure 2—source data 1. Source data for Figure 2C and E.

Figure 2.

Figure 2—figure supplement 1. Dorsal-ventral patterning of the neural tube in Dyrk2-/- mice.

Figure 2—figure supplement 1.

(A) Transverse sections of wild-type and Dyrk2-/- embryos at E10.5 (at the branchial level) were stained for markers of ventral (FOXA2, NKX2.2, OLIG2, and NKX6.1) and dorsal (PAX6) regions. Nuclei were stained with DAPI (blue). (B) qPCR of Gli1, Ptch1, Shh, and Foxf2 in the whole embryos from wild-type and Dyrk2-/- embryos at E9.5. Data are presented as the means ± SEM (n = 3 biological replicates). The statistical significance between wild-type and Dyrk2-/- was determined by the Student’s t-test. (*) p<0.05, (**) p<0.01. (C) In situ hybridization of Ptch1 in the neural tube (left panels) and mandibular arch (right panels) in wild-type and Dyrk2-/- embryos at E10.5 from the transverse and sagittal plane, respectively. Scale bars, 50 µm.
Figure 2—figure supplement 1—source data 1. Source data for Figure 2—figure supplement 1B.