Table 2. List of primer sets.

For genotyping
Gene	Sequence (5'→3')		Accession number
Dyrk2 tm1b-WT	Forward	TGGGTCCAAATGCAAAGAAACGCCA	NC_000076.6
	Reverse	GCTTCTCGTTCCGCACCATCTTCAG
Dyrk2 tm1b-KO	Forward	CCTTCTCCCTCCTCCACTCTGACCCA	NC_000076.6
	Reverse	CCACACCTCCCCCTGAACCTGAAAC
For amplification of the probes for in situ hybridization or Southern blotting
Gene	Sequence (5'→3')		Accession number
Mouse Foxf2	Forward	GAGATTAACCCTCACTAAAGGGAGGTTATGGTGGCCTCGACAT	NM_010225.2
	Reverse	GAGTAATACGACTCACTATAGGGACACACACACCTCCCTTTTCA
Mouse Gli1	Forward	GAGTATTTAGGTGACACTATAGAAGCAGGGAAGAGAGCAGACTG	NM_010296.2
	Reverse	GAGTAATACGACTCACTATAGGGGCTGAGTGTTGTCCAGGTC
Mouse Ptch1	Forward	GAGATTAACCCTCACTAAAGGGACATGGCCTCGGCTGGTAAC	NM_008957.3
	Reverse	GAGTAATACGACTCACTATAGGGTGTACCCATGGCCAACTTCG
Southern for Dyrk2	Forward	CTTCGAATCCTTTTATCCTTCAGGC	NC_000076.6
	Reverse	ACATCATGTTCATTGGTTTTGCTCT
For cloning
Gene	Sequence (5'→3')		Accession number
Mouse Aurka CDS	Forward	GGACTCAGATCTCGAGACATGGCTGTTGAGGGCG	NM_011497.4
	Reverse	GTCGACTGCAGAATTCCTAAGATGATTTGCTGGTTG
Mouse Dyrk2 CDS	Forward	GTGCGCGATCGCCATGTTAACCAGGAAACCTTCGGC	NM_001014390.2
	Reverse	CTCCGTTTAAACGCTAACGAGTTTCGGCAACAC
For real-time PCR
Gene	Sequence (5'→3')		Accession number
Human DYRK2	Forward	GGGGAGAAAACGTCAGTGAA	NM_006482.3
	Reverse	TCTGCGCCAAATTAGTCCTC
Human HPRT1	Forward	GGACTAATTATGGACAGGACTG	NM_000194.3
	Reverse	GCTCTTCAGTCTGATAAAATCTAC
Mouse Aurka	Forward	CACACGTACCAGGAGACTTACAGA	NM_011497.4
	Reverse	AGTCTTGAAATGAGGTCCCTGGCT
Mouse Cdc20	Forward	GAGCTCAAAGGACACACAGC	NM_023223.2
	Reverse	GCCACAACCGTAGAGTCTCA
Mouse Dyrk2	Forward	CTACCACTACAGCCCACACG	NM_001014390.2
	Reverse	TCTGTCCGTGGCTGTTGA
Mouse Foxf2	Forward	AGCATGTCTTCCTACTCGTTG	NM_010225.2
	Reverse	TCTTTCCTGTCGCACACT
Mouse Gli1	Forward	GCACCACATCAACAGTGAGC	NM_010296.2
	Reverse	GCGTCTTGAGGTTTTCAAGG
Mouse Hprt	Forward	CTCATGGACTGATTATGGACAGGAC	NM_013556.2
	Reverse	GCAGGTCAGCAAAGAACTTATAGCC
Mouse Kif2c	Forward	GAGAGCAAGCTGACCCAGG	NM_134471.4
	Reverse	CCTGGTGAGATCATGGCGATC
Mouse Plk1	Forward	CCAAGCACATCAACCCAGTG	NM_011121.4
	Reverse	TGAGGCAGGTAATAGGGAGACG
Mouse Ptch1	Forward	CTCTGGAGCAGATTTCCAAGG	NM_008957.3
	Reverse	TGCCGCAGTTCTTTTGAATG
Mouse Shh	Forward	GTGAAGCTGCGAGTGACCG	NM_009170.3
	Reverse	CCTGGTCGTCAGCCGCCAGCACGC
Mouse Tpx2	Forward	GCGAGGTTGTCAGGTGTGTA	NM_001141977.1
	Reverse	TTGATAAAGTCGGTGGGGGC
Mouse Ube2c	Forward	CTGCTAGGAGAACCCAACATC	NM_026785.2
	Reverse	GCTGGAGACCTGCTTTGAATA