Table 2.

Most relevant B. ovis PA mutants in flagellar loci obtained in this work^a.

B. ovis strainb	Deleted loci and order of deletion	bp deleted
B. ovis PA single mutants (one entire locus deleted)
$\mathrm{B.\; ovis\; \Delta \; flg1}$	Locus I completely deleted	18459
$\mathrm{B.\; ovis\; \Delta \; flg2}$	Locus II completely deleted	6320
$\mathrm{B.\; ovis\; \Delta \; flg3}$	Locus III completely deleted	7736
B. ovis PA double mutants (two entire loci deleted)
B. ovis Δflg1Δflg2	Loci I and II completely deleted	24779
B. ovis Δflg1Δflg3	Loci I and III completely deleted	26195
B. ovis Δflg2Δflg1	Loci II and I completely deleted	24779
B. ovis Δflg2Δflg3	Loci II and III completely deleted	14056
B. ovis Δflg3Δflg1	Loci III and I completely deleted	26195
B. ovis Δflg3Δflg2	Loci III and II completely deleted	14056
B. ovis PA triple mutants (three entire loci deleted)
$\mathrm{B.\; ovis\; \Delta \; flg1\; \Delta \; flg2\; \Delta \; flg3}$	Loci I, II, and III completely deleted	32515
B. ovis Δflg1Δflg3Δflg2	Loci I, III, and II completely deleted	32515
$\mathrm{B.\; ovis\; \Delta \; flg2\; \Delta \; flg1\; \Delta \; flg3}$	Loci II, I, and III completely deleted	32515
B. ovis Δflg2Δflg3Δflg1	Loci II, III, and I completely deleted	32515
B. ovis Δflg3Δflg1Δflg2	Loci III, I, and II completely deleted	32515
$\mathrm{B.\; ovis\; \Delta \; flg3\; \Delta \; flg2\; \Delta \; flg1}$	Loci III, II, and I completely deleted	32515

^aIntermediate strains obtained during mutagenesis are not cited.

^bMutant strains phenotypically characterized in this work are highlighted in blue bold characters. Order of citation of the deleted loci in the strain name corresponds to the order of deletion of each locus (i.e., B. ovis Δflg2Δflg1 was obtained from parental B. ovis PA by deletion of locus II to obtain B. ovis Δflg2 and, then, by deletion of locus I from the Δflg2 single mutant).