Table 4.
Defective flagellar genes in B. melitensis 16M and B. ovis 63/290a.
| Gene identification in the genome of Brucella spp. | Protein | Relevant gene defect(s) when compared to motile Brucella sp. B13-0095b | |
|---|---|---|---|
| B. melitensis 16M | |||
| Locus I | BMEII0151-52 | FliF | 1 nt substitution leading to premature stop codon |
| BMEII0166-67 | FlhA | 1 nt substitution leading to premature stop codon | |
| Locus II | BMEII1085 | FlgA | 18 nt deletion involving start codon and probable ribosome binding site |
| BMEII1084 | FlgI | First 6 nt differ affecting start codon 1 nt deletion leading to frameshift | |
| Locus III | BMEII1113 | FliG | Internal 83 nt deletion with frameshift |
| BMEII1107 | FlgF | 1 nt deletion leading to frameshift | |
| BMEII1106-05 | FliI | 1 nt insertion leading to frameshift | |
| B. ovis 63/290 | |||
| Locus I | BOV_A1052 | FliC | Internal 48 nt in-frame deletion C-terminal 203 nt deletion leading to frameshift and affecting the intergenic FliC-FliF region |
| BOV_A1049 | MotB | 35 nt deletion leading to frameshift | |
| BOV_A1046 | Lytic transglyc. | 1 nt deletion leading to premature stop (frameshift) codon 71 nt deletion | |
| BOV_A1044 | FlgE | Internal 57 nt in-frame deletion | |
| BOV_A1043 | FlgK | Internal 42 nt in-frame deletion Internal 18 nt in-frame deletion | |
| BOV_A1037 | FlhA | Internal 36 nt in-frame deletion | |
| BOV_A1036 | FliR | Internal 48 nt in-frame deletion | |
| Locus II | BOV_A0140 | FlgI | First 6 nt differ affecting start codon 1 nt deletion leading to frameshift |
| Locus III | BOV_A0116 | FliM | 1 nt substitution leading to premature stop codon |
| Chrom. I | BOV_1655 | MotA | Internal 31 nt deletion leading to frameshift |
| BOV_1656 | MotB | Internal 87 nt in-frame deletion | |
a
Compared to the genes of motile Brucella sp. B13-0095 isolated from a Pac-Man frog (Ceratophrys ornata) (13).
b
Relevant defects included in the table are nucleotide deletions, insertions or substitution leading to in-frame deletions, frameshift or premature stop codons. Nucleotide substituions that do not introduce premature stop codons are not considered as relevant defects.