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. 2020 Jul 31;8:891. doi: 10.3389/fbioe.2020.00891

FIGURE 6.

FIGURE 6

Effect of milk extracellular vesicle treatment in the osteocytes. (A) Number of osteoblasts (N.Ob) per bone perimeter (B.Pm) and representative pictures of osteoblasts in the trabecular bone (400×). Yellow arrows indicate osteoblasts. (B) Osteocyte density and representative pictures of osteocytes in the trabecular bone (400×). (C) mRNA expression of sclerostin in the femur. Analyses were performed in mice (i) pseudo-operated (SHAM) or (ii) ovariectomized (OVX), (iii) non-treated (control), or (iv) treated with milk extracellular vesicles (mEVs). Values are means ± SEM of 6–8 animals per group. * vs. respective SHAM group and # vs. non-treated group. P < 0.05. Osteocyte MLO-Y4 cell line (D) proliferation assay and (E) Fgf2 mRNA expression. (F) Apoptosis assay of osteocyte cell line treated with dexamethasone (DM) (10 μM) and mEVs (10 and 100 μg/ml). mRNA expression of (G) Bax/Bcl2 ratio and (H) Rankl, Opg, and Rankl/Opg ratio in osteocyte culture treated with mEVs (100 μg/ml) (n = 4). Bars represent the mean ± SEM. * vs. PBS. P < 0.05.