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. 2020 Aug 6;10:13212. doi: 10.1038/s41598-020-69984-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mycobacterium bovis BCG infection induced high expression of mRNA of IL-35 subsets Ebi3 and p35 in mononuclear cells in the peripheral blood and in immune tissues in mice. PBMCs and mononuclear cells from lung (LMC), spleen, and bone marrow were extracted from Mycobacterium bovis BCG-infected mice (n = 6). Then, the cells were assayed for IL-35 subsets p35 and Ebi3 mRNA expression using real-time PCR. (A–D) Relative mRNA expression of IL-35 subset p35 in the peripheral blood (A), lung (B), spleen (C), and bone marrow (D). (E–H) Relative mRNA expression of IL-35 subset Ebi3 in the peripheral blood (E), lung (F), spleen (G), and bone marrow (H). The P value is shown in each column. *P < 0.05; **P < 0.01; ***P < 0.001.