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. 2020 Aug 6;10:13303. doi: 10.1038/s41598-020-70145-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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a Genes classified as essential in double deletions. The connected pairs means that their conjunct deletion resulted in the deletion of the cell division reaction. They are grouped by colors respective to their functional groups: cross-membrane ion transporters (green), protein degradation (yellow), and folate metabolism (blue). b Gene essentiality prediction of randomly rewired networks with probability p from the original M. genitalium whole-cell biochemical network. Each rewiring rate accounts for 50 replicates.