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. 2020 Aug 6;11:3912. doi: 10.1038/s41467-020-17704-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Concatenated FlowSOM analysis of naïve brain vs. tumor-bearing brain showing seven metaclusters (representative plot from two independent experiments with n = 3 mice/group). b Expression of CD11b, CD45 and CX3CR1 in viSNE clusters of naïve vs. tumor-bearing brain. c Heatmap showing expression profile of metaclusters in concatenated samples. d Percent population in each metacluster of naïve vs. tumor-bearing brain. Data represented as average ± SE for n = 3 mice/group. Data representative of two independent experiments. Two-sided Student’s t test with Holm–Sidak corrections for multiple comparisons was applied. *p ≤ 0.05; **p ≤ 0.005; ***p ≤ 0.0007. e Representative flow plot of resting and activated microglia in naïve vs. tumor-bearing brain. Microglia was gated as CD11b low/CD45 low which was further gated for MHC-II high/CX3CR1 high (activated microglia) and MHC-II low/CX3CR1 high (resting microglia).