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. 2020 Jul 31;11:1705. doi: 10.3389/fmicb.2020.01705

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Copyright © 2020 Grande, Sisto, Puca, Carradori, Ronci, Aceto, Muraro, Mincione and Scotti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative H. pylori biofilms stained with Live/Dead kit and analyzed using fluorescence microscopy. The green fluorescence indicates the live cells, whereas the red fluorescence indicates the dead cells or cells with a damaged cell wall. Panels (a,c,e,g) show the untreated H. pylori biofilms, while panels (b,d,f,h) show H. pylori biofilms treated with SUNCs at MBEC concentrations of 1.28 mg/L for H. pylori strains ATCC 43504 and 23, 0.96 mg/L for 110 R, and 0.64 mg/L for F40/499. Scale bar: 5 μm.