Table 2.

Sequences of primers used for real-time PCR.

Target	Accession number	Sense and Antisense primer	PCR product (bp)
GAPDH	AF017079	F:5′GAAGGTCGGAGTGAACGGAT3′	149
		R:5′CATGGGTAGAATCATACTGGAACA3′
Occludin	NM_001163647.1	F:5′ATGCTTTCTCAGCCAGCGTA3′	176
		R:5′AAGGTTCCATAGCCTCGGTC3′
ZO-1	XM003353439.1	F:5′GAGGATGGTCACACCGTGGT3′	169
		R:5′GGAGGATGCTGTTGTCTCGG3′
Claudin-1	NM_001161635.1	F:5′GGACTAATAGCCATCTTTGT3′	88
		R:5′CAGCCATCCGCATCTTCT3′
IL-1β	NM_214055.1	F:5′ACCTGGACCTTGGTTCTC3′	124
		R:5′GGATTCTTCATCGGCTTC3′
TNF-α	NM_214022.1	F:5′ACGCTCTTCTGCCTACTGC3′	162
		R:5′TCCCTCGGCTTTGACATT3′
IL-10	NM_214041.1	F:5′CACTGCTCTATTGCCTGATCTTCC3′	136
		R:5′AAACTCTTCACTGGGCCGAAG3′
IL-6	NM_214399.1	F:5′TTCAGTCCAGTCGCCTTCT3′	91
		R:5′GTGGCATCACCTTTGGCATCTTCTT3′