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. 2020 Jul 31;11:855. doi: 10.3389/fgene.2020.00855

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Copyright © 2020 Velegzhaninov, Belykh, Rasova, Pylina, Shadrin and Klokov.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of the overexpression of the RPA1 gene on the resistance of HEK293T cells to ionizing radiation and the proliferation rate. (A,B) The levels of mRNA (qRT-PCR) and protein (western blotting) of RPA1 48 h after cotransfection of cells with the plasmids encoding the dCas9-VPR activator and guide RNAs (three biological replicates per group in each analysis). (C) The proportion of surviving CFU after exposure to γ-radiation at 3 Gy (12 replicates per group). (D) The average number of cells per coloniy (25 randomly selected colonies per group). *, differences with the TC group are significant at p < 0.05 (Student’s t-test).