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Comparison of Ct values obtained by TaqMan, SYBR Green, and one-step/two-step RT-PCR assays. a Ct values of 63 clinical samples detected with TaqMan and SYBR Green RT-qPCR. b, c Agarose gels of amplified fragments by one-step RT-PCR and two-step PCR assays, respectively, of 14 representatives’ clinical samples. The 600-bp marker in the ladder (LD) is indicated in the picture. The arrow points to the fragment of 113 base pairs (bp) amplified with SARS-CoV-2-specific E gene primers
