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. 2020 Jun 30;22(3):1759–1766. doi: 10.3892/mmr.2020.11291

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Copyright: © Xue et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — PI3k/Akt/eNOS pathway expression. (A) PI3K mRNA expression from atrium tissue normalized to β-actin. (B) Akt mRNA expression from atrium tissue normalized to β-actin. (C) eNOS mRNA expression from atrium tissue normalized to β-actin. (D) Representative western blotting bands of PI3K/Akt/eNOS pathway proteins. (E) PI3K protein expression level normalized to β-actin. (F) p-Akt/Akt protein level ratio. (G) p-eNOS/eNOS protein level ratio. n=20 for each group. Data are presented as mean ± SD. **P<0.01, ***P<0.001 and ****P<0.0001 vs. the control group; ^#P<0.05, ^##P<0.01, ^###P<0.001 and ^####P<0.0001 vs. the DM group. Fold-change indicates the value compared with the control group. p-, phosphorylated; eNOS, endothelial NOS; DM, diabetes mellitus; H₂S, hydrogen sulfide; STZ, streptozotocin.