Figure 3.

Relative expression levels of Notch genes in hepatic cell lines. LX2, Huh7 and MIHA cells were treated without PA (CTL) or 0.25 mM palmitic acid, and RT-qPCR was performed to assess expression levels of Notch1, −2, −3, −4, Jagged1, Jagged2, DLL1, DLL3, Hey1 and Hes1 following various durations of treatment (12, 24, 36 or 48 h). Data are presented as the mean ± SD. *P<0.05. CTL, control; DLL, delta-like ligand; Hey1, hes-related family bHLH transcription factor with YRPW motif 1; Hes1, hairy and enhancer of split-1.