Figure 2.

Lentivirus-delivered siRNA silencing specifically inhibits the expression of RPL32 at the mRNA and protein levels. (A) Infection efficiency of the lentivirus was monitored by detecting GFP expression after 3 days of transduction (magnification, ×100). (B) Reverse transcription-quantitative PCR demonstrated that the RPL32 mRNA expression was significantly decreased in 1315-KD cells. (C) Western blot analysis identified that (D) RPL32 protein expression was significantly decreased in 1315-KD cells. 1315-CON and SUM 1315 cells were stably transfected with negative control lentivirus, while 1315-KD and SUM 1315 cells were stably transfected with RPL32 siRNA lentivirus. *P<0.05 vs. 1315-CON. si, small interfering; RPL32, ribosomal protein L32; GFP, green fluorescent protein; CON, control; KD, knockdown.