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. 2020 Jul 8;22(3):2373–2385. doi: 10.3892/mmr.2020.11312

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effect of Alda-1 on 4-HNE accumulation and oxidative stress in rats with hepatic IR injury. (A) Western blotting was used to analyze the formation of 4-HNE protein adducts in liver tissue after 6 and 24 h of reperfusion. Relative expression was normalized to the β-actin loading control. (B) Densitometric semi-quantification of 4-HNE protein adduct formation from part (A). (C) Serum levels of 4-HNE after 6 and 24 h of reperfusion. (D) 4-HNE, (E) ROS levels and (F) MDA levels in liver tissue after 6 and 24 h of reperfusion. Data are presented as the mean ± SD. n=6. *P<0.05 vs. the sham group; ^#P<0.05 vs. the IR group; ^$P<0.05 vs. the IR-Daidzin group. RP, reperfusion; 4-HNE, 4-HNE, 4-hydroxy-2-nonenal; ROS, reactive oxygen species; MDA, malondialdehyde; IR, ischemia/reperfusion.