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Alda-1 pretreatment reduces the distribution of HMGB1, TLR4 and release of IL-6. (A) Immunohistochemical staining was used to determine the expression levels of HMGB1 and TLR4 after 24 h of reperfusion. Magnification, × 200; scale bar, 100 µm. (B) Immunofluorescence images showing IL-6 expression levels after 24 h of reperfusion. Magnification, ×400; scale bar, 50 µm. n=6. HMGB1, high mobility group box 1; TLR4, toll-like receptor 4; IL, interleukin; IR, ischemia/reperfusion.
