Figure 2.

Binding of C. difficile SLPs to cells. CHO-K1 cells were exposed to various concentrations of SLPs (0–20 μg/ml) and incubated at 4°C for 2 h. The cells were probed with the anti-SLP antibody at 4°C for 2 h, followed by incubation with FITC-conjugated goat anti-rabbit IgG. The binding activity of SLPs was assessed using flow cytometry to detect FITC fluorescence intensity. MCF, mean channel fluorescence.