Figure 1.

Effects of CaSR on Col-I/II and MMPs in CFs treated with HG. CFs in the control group were cultured with 5.5 mM glucose and CFs in the HG group were cultured with 40 mM glucose with or without 5 µM R568 or 3 µM Calhex231. (A and B) CaSR, α-SMA, Col-I, Col-III, MMP2 and MMP9 expression was determined by western blotting. (C) Concentration of Col-I and Col-III in the CFs supernatant determined by ELISA. *P<0.05 vs. control; ^#P<0.05 vs. HG. n≥3. CaSR, calcium sensing receptor; CFs, cardiac fibroblasts; HG, high glucose; α-SMA, α-smooth muscle actin; Col-I, collagen I; Col-III, collagen III; MMP2, matrix metalloproteinase 2; MMP9, matrix metalloproteinase 9.