Figure 5.

MR antagonism suppresses H₂O₂-induced cardiac aging and mitochondrial dysfunction. (A) Immunoblotting and semi-quantification of p16, p21, p53 and NADP subunits, p47-phox, p67-phox and gp91-phox expression in H9C2 cells. Protein expression levels were normalized to GAPDH. (B) ROS levels detected by DHE staining in H9C2 cells following different treatments (magnification, ×100; scale bar, 100 µm). (C) SOD-1, SOD-2 and PGC-1α protein expression levels detected by western blotting. Data are representative of three experiments, n=3. **P<0.01 and ***P<0.001 vs. control group; ^#P<0.05 vs. H₂O₂ group. DHE, dihydroethidium; MR, mineralocorticoid receptors; PGC-1α, peroxisome proliferator-activated receptor γ coactivator-1α; ROS, reactive oxygen species; SOD, superoxide dismutase; Eple, eplerenone.