Figure 4.

Effect of QUE on the phosphorylation of IκB-α and the nuclear translocation of NF-κB in LPS-induced RAW264.7 macrophages. Cells were pre-treated for 2 h with various concentrations (1, 10 or 100 µM) of QUE, and induced for 30 min with 1 µg/ml of LPS. (A) Whole-cell lysates were prepared and subjected to western blot analysis with anti-IκB-α and anti-p-IκB-α antibodies. β-actin was used as a loading control. (B) Cytosolic and nuclear extracts were prepared and subjected to western blot analysis with anti-NF-κB p65 antibody. Histone H3 and GAPDH were used as loading controls. ^###P<0.001 vs. non-treated group; **P<0.01 and ***P<0.001 vs. LPS-treated group. NS, not significant; QUE, quercetogetin; LPS, lipopolysaccharide; nuclear, nuclear extracts; cytoplasmic, cytoplasmic extracts; IκB-α, NF-κB inhibitor α; p-, phosphorylated.