Table I.
Primer sequences for PCR.
| Gene name | Genera | Primer sequences (5′-3′) |
|---|---|---|
| ChREBPa | Mouse | F: CGACACTCACCCACCTCTTC |
| R: TTGTTCAGCCGGATCTTGTC | ||
| ACC | Mouse | F: CTTCGGGGTGGTTCTTGGGTTGTG |
| R: CCTGCATCCGGCCTGGTGTG | ||
| FASN | Mouse | F: ATCCTGGAACGAGAACACGATCT |
| R: AGAGACGTGTCACTCCTGGACTT | ||
| TGF-β | Mouse | F: GCAGTGGCTGAACCAAGGA |
| R: AGCAGTGAGCGCTGAATCG | ||
| Collagen IV | Mouse | F: CAACCTGGACGCCATCAAG |
| R: CAGACGGCTGAGTAGGGAACA | ||
| ACTB | Mouse | F: CAGCCTTCCTTCTTGGGTAT |
| R: TGGCATAGAGGTCTTTACGG | ||
| ChREBP | Human | F: AGTGCTTGAGCCTGGCCTAC |
| R: TTGTTCAGGCGGATCTTGTC | ||
| LXRα | Human | F: AGAAGAACAGATCCGCCTGAAG |
| R: GGCAAGGATGTGGCATGAG | ||
| SREBP1 | Human | F: GGGCGTGAAGACTGAGGTG |
| R: CTGCTGCCAAGGGACAAG | ||
| TRAK2 | Human | F: ATGAGTCAATCCCAGAATGCAA |
| R: TCAGTCCTCCTTCAGGACACC | ||
| ABCA1 | Human | F: TACAGCCAGAAAGACACCAG |
| R: CACAGTAGACTTTGGGAGAG | ||
| ABCG1 | Human | F: TCGGTGGATGAGGTGGTG |
| R: TGGGCTTCCGTGAGGTTA | ||
| PKLR | Human | F: GTGGACATCGTCTTTGCCT |
| R: TCTTGATGCCGTGTCCTTC | ||
| FASN | Human | F: CGCTCGGCATGGCTATCT |
| R: CTCGTTGAAGAACGCATCCA | ||
| ACC | Human | F: GGATGGTGTTCACTCGGTAATAG |
| R: GGGTGATATGTGCTGCGTCAT | ||
| ACTB | Human | F: TGGATCAGCAAGCAGGAGTATG |
| R: GCATTTGCGGTGGACGAT |
chREBP, carbohydrate-responsive element-binding protein; ACC, acetyl-CoA carboxylase; FASN, fatty acid synthase; TGF-β, transforming growth factor-β; ACTB, β-actin; LXRa, liver X receptor α; SREBP1, sterol regulatory element-binding 1; TRAK2, trafficking kinesin protein 2; ABCA1, ATP-binding cassette subfamily A member 1; ABCG1, ATP-binding cassette subfamily G member 1; PKLR, pyruvate kinase L/R.