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. 2020 Jul 10;22(3):2460–2468. doi: 10.3892/mmr.2020.11334

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Effect of GAS5 on chemosensitivity in MCF-7 cells. (A) CCK-8 assay was performed to detect chemosensitivity in MCF-7 after GAS5 overexpression. (B) After 24-h treatment of 2 µM Cisplatin, the apoptotic rate of cells was analyzed using flow cytometry after Annexin V-FITC and PI double staining. *P<0.05 vs. the GAS5-1/2+3-MA group, ^#P<0.05 vs. Vector group. GAS5, growth arrest-specific 5; 3-MA, 3-methyladenine; IC, inhibitory concentration; Annexin V+/PI-, early apoptotic cells; Annexin V+/PI+, late apoptotic cells.