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. 2020 Jun 18;22(3):1932–1948. doi: 10.3892/mmr.2020.11259

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Copyright: © Ramírez-Ricardo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — EVs isolated from patients with breast cancer mediate secretion of gelatinases. (A) MDA-MB-231 cells were incubated for various times with EVs from three healthy women and EVs from three patients with breast cancer, and conditioned media were collected. (B) MDA-MB-231 cells were incubated for 24 h with EVs from three healthy women and three patients with breast cancer. Gelatinase secretion was analyzed by gelatin-substrate gels, and positive controls of MMP-2 (EtOH) and MMP-9 (PDB) secretions were included. (C and D) Densitometric analysis of MMP-9 and MMP-2 secretion. Data are presented as the mean ± SD, and are expressed as fold of MMP-2 or MMP-9 secretion above Ctrl and Ctrl EVs. *P<0.05, **P<0.01 as indicated. Ctrl, control; EVs, extracellular vesicles; Ctrl EVs, EV fractions obtained from healthy women; BC EVs, EV fractions obtained from women with breast cancer; MMP, matrix metalloproteinase; EtOH, ethanol; PBD, phorbol-12,13-dibutyrate.