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. 2020 Jun 26;22(3):2043–2051. doi: 10.3892/mmr.2020.11276

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Copyright: © Shi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — In vitro characteristics of cultured PDLSCs. Scale bars=200 µm. (A) PDLSCs grew around periodontal ligament tissues five days after incubation. (B) PDLSCs reached confluence seven days after incubation. (C) Entire plate view of colony-forming PDLSCs, which were plated at a low density and cultured for 7 days before staining with crystal violet. (D) Cell clusters derived from PDLSCs with typical fibroblast-like morphology seven days after incubation. (E) Alizarin Red-S staining 14 days after incubation. (F) Oil Red O staining four weeks after incubation. PDLSC, periodontal ligament stem cell.