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. 2020 Jul 31;10:1166. doi: 10.3389/fonc.2020.01166

Figure 2.

Figure 2

Loss of function of YTHDC2 enhances radiosensitivity of NPC cell in vitro. (A) Demonstration of shRNA efficiency in down-regulation of YTHDC2 in CNE2-IRR and HK1-IRR cells. (B) CCK8 assays of CNE2-IRR and HK1-IRR cells with knockdown of YTHDC 2 with or without irradiation treatment. Data are presented as mean ± SD from n = 3. ***P < 0.001, student's t-test. (C) Images from soft agar colony formation assay in CNE2-IRR and HK1-IRR cells with YTHDC2 knockdown after expose to radiation were shown in 2 different magnifications (40×and 200×). Data are presented as mean ± SD from n = 4. (D) Quantitative analyses of colony size (50–100 μm and >100 μm) in CNE2-IRR and HK1-IRR cells with YTHDC2 knocking down after exposure to radiation. (E) Survival rate in CNE2-IRR cells expose to 0, 2, 4, 6, 8 Gy radiation after YTHDC2 was knocked down. Data are presented as mean ± SD from n = 3. **P < 0.01, ***P < 0.001, student's t-test. (F) The apoptosis assay of CNE2-IRR with YTHDC2 knockdown after 0 or 6 Gy radiation by flow cytometric analysis (left) and the statistical analysis of the data (right). Data are presented as mean ± SD from n = 3, student's t-test.