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. 2020 Jul 31;11:958. doi: 10.3389/fphys.2020.00958

FIGURE 2.

FIGURE 2

Increased erythroblast binding to VCAM-1 and fibronectin after Yoda1 mediated PIEZO1 stimulation. (A,B) Erythroblasts were treated or not with 1 μM of Yoda1 for 10 min and subjected to a flow assay as described in Materials and Methods (7.5 ml/h flow rate). Adhesion of erythroblasts to VCAM (A) or fibronectin (B) was quantified using imageJ (n = 9 pictures of different donors; data depicted as mean ± SD; * * **P < 0.0001; C,D) Erythroblasts were pre-treated with anti-α4, anti-α5, or anti-β1 and stimulated or not with 1 μM Yoda1 (10 min) as indicated and flowed over a surface coated with VCAM (C) or fibronectin (D; data depicted as mean ± SD mean n = 9 images was used to calculate statistic; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and * * **P < 0.0001).