Figure 2.

Effect of punicalagin on the apoptosis of human osteosarcoma cell lines. Cells were treated with 100 µM punicalagin for 48 h, then analyzed using annexin V and propidium iodide double staining. Punicalagin treatment induced consistent changes seen as increased frequencies of both early (FITChighPIlow) and late (FITChighPIhigh) apoptotic cells in (A) U2OS, (B) MG63 and (C) SaOS2 cells. The representative diagrams of flow cytometry analyses were displayed. Data are presented as the mean ± SEM of three independent experiments. **P<0.01, vs. control group. PE, phycoerythrin; FITC, Fluorescein isothiocyanate; Ctrl, control.