Figure 8.

Effect of PMG on the expression of total p-Erk/Erk in the prefrontal cortex was detected by Western blotting. (a) Representative Western blot showing the levels of total p-Erk/Erk. (b) Relative density of total p-Erk/Erk. Data are presented as mean ± SEM (n = 6/group). ^aaap value < 0.001; compared between naïve control which received a normal diet and vehicle and MetS+MCAO rats which received HCHF, MCAO, and vehicle. ^bbbp value < 0.001; compared between sham rats which received HCHF, sham operation, and vehicle and MetS+MCAO rats which received HCHF, MCAO, and vehicle. ^∗p value < 0.05, ^∗∗p value < 0.01, and ^∗∗∗p value < 0.001; compared to MetS+MCAO rats which received HCHF, MCAO, and vehicle. ND: normal diet; HCHF: high-carbohydrate high-fat diet; MetS: metabolic syndrome; MCAO: middle cerebral artery occlusion; Vitamin C: vitamin C at a dose of 250 mg·kg⁻¹ BW; Donepezil: donepezil at a dose of 3 mg·kg⁻¹ BW; PMG50, PMG100, and PMG200: the phytosomes containing the combined extract of mulberry fruit and ginger at doses of 50, 100, and 200 mg·kg⁻¹ BW, respectively.