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. 2020 Jul 3;25(13):3045. doi: 10.3390/molecules25133045

Figure 7.

Figure 7

The synergistic effect of the alkaloids combination is related to the reactive oxygen species (ROS). Cells were treated for 24 h with PL at concentrations 1–8 µM; SAN at doses of 0.25, 0.5, 1, 1.5 and 2 µM and their combination in ratio 4:1. To inhibit ROS induction, A549 cells were preincubated for 2 h with 5-mMN-acetyl-l-cysteine (NAC) and then treated with alkaloids for 24 h. (A) The results obtained are presented as the percentage of ROS-positive cells relative to the sample size. (B) The comparison of results from the AV/PI assay for cells without (A549) and preincubated with NAC (A549 + NAC). Statistical significance was marked with * for the comparison between A549 cells without and with the preincubation with NAC, # for A549 cells without incubation with the ROS inhibitor in comparison to the CTRL and $ A549 cells with the addition NAC compared to untreated cells (p < 0.05; two-way ANOVA). (C) Cell cycle analysis for A549 and A549 + NAC. Data represent the mean ± SD obtained from 4 independent replicates (n = 4). Statistically significant differences in comparison to the untreated cells were marked as * (p < 0.05; two-way ANOVA).