Table 4.
Serological results of certain serum samples taken during the animal trial using the ID Screen Capripox Double Antigen ELISA (ID.vet), as well as the serum neutralization test (SNT).
| Group A 3.8 × 102 CCID50/Animal | Group B 2.4 × 104 CCID50/Animal | Group C 3.3 × 106 CCID50/Animal | Group D 3.8 × 107 CCID50/Animal | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cattle _Dpi | ELISA S/P% | SNT Titer | Cattle_Dpi | ELISA S/P% | SNT Titer | Cattle_Dpi | ELISA S/P% | SNT Titer | Cattle_Dpi | ELISA S/P% | SNT Titer |
| R/279_−1 | −1 | n.d. | R/888_−1 | 0 | n.d. | R/277_−1 | −1 | n.d. | R/276_−1 | −1 | n.d. |
| R/279_14 | 0 | n.d. | R/888_11 | −1 | <1:10 | R/277_13 | 5 | <1:10 | R/276_14 | 8 | n.d. |
| R/279_17 | −1 | n.d. | R/276_17 | 11 | n.d. | ||||||
| R/279_21 | 0 | <1:10 | R/276_21 | 11 | 1:13 | ||||||
| R/279_28 | 0 | <1:10 | R/276_28 | 41 | 1:160 | ||||||
| R/278_−1 | 0 | n.d. | R/857_−1 | 1 | n.d. | R/283_−1 | −1 | n.d. | R/282_−1 | −1 | n.d. |
| R/278_14 | 0 | n.d. | R/857_14 | 0 | n.d. | R/283_11 | −1 | <1:10 | R/282_14 | 4 | n.d. |
| R/278_17 | 0 | n.d. | R/857_17 | 0 | n.d. | R/282_17 | 14 | 1:16 | |||
| R/278_21 | −1 | <1:10 | R/857_21 | 1 | <1:10 | R/282_21 | 22 | 1:32 | |||
| R/278_28 | 3 | <1:10 | R/857_28 | 6 | ≤1:13 | R/282_28 | 43 | 1:128 | |||
| R/280_−1 | −2 | n.d. | R/891_−1 | 0 | n.d. | R/284_−1 | −1 | n.d. | R/981_−1 | 8 | n.d. |
| R/280_13 | 1 | <1:10 | R/891_14 | −1 | n.d. | R/284_14 | 11 | n.d. | R/981_11 | 14 | <1:10 |
| R/891_17 | 1 | n.d. | R/284_17 | 11 | n.d. | ||||||
| R/891_21 | 0 | <1:10 | R/284_21 | 4 | 1:16 | ||||||
| R/891_28 | 128 | 1:40 | R/284_28 | 57 | 1:50 | ||||||
| R/824_−1 | 0 | n.d. | R/829_−1 | −1 | n.d. | R/921_−1 | −1 | n.d. | R/860_−1 | 1 | n.d. |
| R/824_14 | 3 | n.d. | R/829_13 | 8 | <1:10 | R/921_14 | 4 | <1:10 | R/860_14 | 26 | 1:16 |
| R/824_17 | 0 | <1:10 | R/921_17 | 13 | <1:10 | R/860_17 | 59 | 1:128 | |||
| R/824_21 | −1 | <1:10 | R/921_21 | 25 | 1:32 | R/860_21 | 98 | 1:128 | |||
| R/921_28 | 67 | 1:80 | R/860_28 | 163 | 1:256 | ||||||
| R/915_−1 | −1 | n.d. | R/984_−1 | −1 | n.d. | R/841_−1 | 2 | n.d. | R/893_−1 | 12 | n.d. |
| R/915_14 | −1 | n.d. | R/984_13 | 5 | <1:10 | R/841_11 | 0 | <1:10 | R/893_14 | 7 | <1:10 |
| R/915_17 | −1 | n.d. | R/893_17 | 16 | 1:50 | ||||||
| R/915_21 | −2 | <1:10 | R/893_21 | 9 | 1:80 | ||||||
| R/915_28 | 3 | ≤1:13 | R/893_28 | 36 | 1:50 | ||||||
| R/203_−1 | −1 | n.d. | R/990_−1 | 2 | n.d. | R/989_−1 | 2 | n.d. | R/988_−1 | 1 | n.d. |
| R/203_14 | 0 | n.d. | R/990_14 | 6 | n.d. | R/989_11 | 2 | <1:10 | R/988_11 | 8 | <1:10 |
| R/203_17 | 0 | n.d. | R/990_17 | 0 | n.d. | ||||||
| R/203_21 | −1 | <1:10 | R/990_21 | 7 | <1:10 | ||||||
| R/203_28 | 2 | <1:10 | R/990_28 | 3 | 1:20 | ||||||
An S/P% ratio ≥ 30 was defined as positive in the DA ELISA, whereas a neutralizing titer of ≥ 1:20 in the SNT displays a positive result; n.d. means not determined.