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. 2020 Jul 2;25(13):3028. doi: 10.3390/molecules25133028

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Workflow of proteomic analysis of Emiliania huxleyi (E. huxleyi) using three-dimensional liquid chromatography (3D-LC) system. The proteome digest of E. huxleyi (CCMP371) cell lysates was fractionated into 14 strong cation exchange (SCX) fractions. Each of them was further separated into 16 fractions using high-pH reversed-phase liquid chromatography (HpH RPLC). A set of 16 fractions generated from one SCX fraction was concatenated into five final fractions (1-6-11, 2-7-12, 3-8-13, 4-9-14, and 5-10-15-16). Overall, 70 fractions were generated from the original proteome digest of E. huxleyi cell lysates for liquid chromatography -tandem mass spectrometry (LC-MS/MS) analyses.