Skip to main content
. 2020 Jul 14;21(9):1149–1166. doi: 10.1111/mpp.12968

Figure 1.

Figure 1

LsGRP1 silencing increases lily susceptibility to grey mould caused by Botrytis elliptica. (a) The morphology of virus vector‐untreated lily (Un‐VIGS) and virus vector‐treated LsGRP1‐silenced (VIGS‐LsGRP1) and VIGS‐control (VIGS‐CK) lilies at 8 weeks old (upper panel). Agrobacterium‐mediated tobacco rattle virus (TRV) vector‐induced gene silencing was performed 5 weeks before observation. The shoot length and expanding leaf number of 7‐week‐old lily plants are shown in lower panel. The levels of TRV vector, LsGRP1 expression (b) and LsGRP1 accumulation (c) in leaves were detected by quantitative reverse transcription PCR and western blot analysis, respectively. (c) LsGRP1 of 24‐, 16‐, and 14‐Da detected by LsGRP1N antibody (α‐LsGRP1) indicated by arrows from top to bottom, and RuBisCO large subunit (RbcL) stained by Coomassie brilliant blue was used as a loading control. The LsGRP1‐silenced and VIGS‐control leaves were detached and droplet‐ (20 μl in [d] and [g]) or spray‐inoculated ([e] and [f]) with spore suspension of B. elliptica at 5 × 104 spores/ml. (d) Relative B. elliptica biomass was detected by quantitative PCR at 1 day post‐inoculation (dpi). (e) Symptom at 3 dpi (left panel) and trypan blue‐stained host cell death at 7 dpi (middle panel). Necrotic lesions and hypersensitive responses (HR) are indicated by blue and purple arrows, respectively. The proportion of necrotic area at 7 dpi labelled by trypan blue was quantified (right panel). (f) Necrotic lesion in LsGRP1‐silenced leaves and HR in VIGS‐control leaves were microscopically observed at 2 dpi after trypan blue staining. B. elliptica is indicated by arrows. Bar: 2 cm in (e); 200 μm in (f). (g) In planta fungal sporulation occurred in LsGRP1‐silenced leaves but not VIGS‐control leaves at 10 dpi. Data represent the mean ± SD from four, four, three, five, and four biological replicates in (a), (b), (d), (e), and (g), respectively. Statistical analysis was performed using one‐way analysis of variance (ANOVA) followed by Fisher's least significant difference (LSD) test (p < .05)