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. 2020 Jul 8;21(9):1131–1148. doi: 10.1111/mpp.12967

Figure 4.

Figure 4

Suppression of RXLR‐triggered immunity by PaRXLR40. Suppression of (a) Avr3a and R3a‐triggered cell death and (b) PaRXLR24‐triggered cell death by PaRXLR40 on 5‐week‐old Nicotiana benthamiana. Avr3a, R3a, and PaRXLR were labelled with GFP, HA, and FLAG tags, respectively. Agrobacterium tumefaciens carrying cell death elicitors were infiltrated 24 hr after infiltration of Phytophthora agathidicida RXLR effector candidate PaRXLR40, or the negative suppression control, PaRXLR1. Photographs with visible light (top) and UV (bottom) were taken 7 days post‐infiltration of cell death elicitors. Suppression is shown by lack of cell death at infiltration spots (a) 7 and 9 and (b) 5 and 7. The experiment was repeated three times with consistent results. (c) and (d) Protein immunoblots of total proteins extracted from N. benthamiana leaves collected 3 days post‐infiltration confirmed the presence of elicitors and PaRXLR effector candidates. Representative protein loading is shown by Ponceau staining (PS).