Figure 7.

Identification of NBS‐LRRs required for PaRXLR24‐triggered cell death. Agrobacterium‐mediated screening was used to identify NBS‐LRRs required for PaRXLR24‐triggered cell death in 5‐week‐old Nicotiana benthamiana. Hairpin constructs (HP) that targeted individual NBS‐LRRs were infiltrated 48 hr before PaRXLR24. Photographs were taken 7 days after PaRXLR24 infiltration. (a) Leaves showing examples of suppression scoring, with no suppression (red), partial suppression (yellow), and strong suppression (green) of PaRXLR24‐triggered cell death by hairpin constructs. (b) Percentages of infiltration spots showing different levels of suppression by individual hairpin constructs that silenced NBS‐LRRs from hairpin pools 7 and 14. Numbers of infiltration sites counted are shown above each bar. NBS‐LRRs targeted by hairpin constructs HP7‐1, HP7‐2, HP14‐6, and HP14‐8 suppressed PaRXLR24‐triggered cell death more than 50% of the time