Table 2.
Features of the nine RXLR effector candidates from Phytophthora agathidicida that induced or suppressed cell death in Nicotiana spp.
| Name | Best BLAST hit | E‐value | GenBank ID | % amino acid identity | Cell death‐triggering activity f | Suppressed RXLR‐triggered immunity g | |
|---|---|---|---|---|---|---|---|
| Nt | Nb | ||||||
| PaRXLR2 | Phytophthora cactorum hypothetical protein a | 2.0E−38 | RAW43538.1 | 53 | Weak | No | No |
| PaRXLR5 b | Phytophthora parasitica P1569 hypothetical protein a | 6.0E−22 | ETI36999.1 | 45 | Strong | No | No |
| PaRXLR12 | Phytophthora megakarya hypothetical protein | 4.0E−21 | OWZ07307.1 | 41 | Weak | No | No |
| PaRXLR24 c | P. parasitica pPE4 a | 3.0E−48 | XP_008889734.1 | 65 | Strong | Strong | No |
| PaRXLR29 | P. parasitica hypothetical protein | 9.0E−19 | XP_008894466.1 | 38 | Weak | No | No |
| PaRXLR52 d | Phytophthora sojae hypothetical protein a | 7.0E−56 | XP_009533161.1 | 62 | Weak | No | No |
| PaRXLR54 | Phytophthora palmivora avirulence protein | 5.0E−100 | POM79043.1 | 45 | Strong | Strong | No |
| PaRXLR66 e | P. megakarya RXLR protein | 2.0E−27 | OWZ17774.1 | 37 | Weak | No | No |
| PaRXLR40 | P. palmivora RXLR protein a | 1.0E−15 | POM65748.1 | 38 | No | No | Yes |
a
Reciprocal top BLAST hit.
b
Ortholog of P. nicotianae Avh8 (KUG01203.1). E value 2e−21, 43.4% amino acid identity.
c
Ortholog of P. sojae Avh238 (AEK81002.1). E value 8e−28, 46.4% amino acid identity.
d
Ortholog of P. palmivora Avr1b‐1 (POM62647.1). E value 4e−39, 60.7% amino acid identity.
e
Only PaRXLR66 showed single nucleotide polymorphism variation among 14 P. agathidicida genomes.
f
RXLR triggered cell death on N. tabacum (Nt) or N. benthamiana (Nb).
g
RXLR suppressed Avr3a‐R3a and PaRXLR24‐triggered cell death on N. benthamiana.