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. 2020 Jul 21;20(14):4050. doi: 10.3390/s20144050

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A). Determination of CYP1A2 enzymatic activity in yRD⁺ and yRD⁻ yeast cells using 3-cyano-7-ethoxycoumarin (CEC) as a substrate. CYP1A2 gene expression is driven by the ADH2 promoter (ADH2p). The results show that the CPR (in this case, the yeast reductase, yRD) is essential for CYP450 activity. (B) Determination of CYP1A2 enzymatic activity in yRD⁺:: GAPDH-1A2 and yRD⁻:: GAPDH-1A2 yeast cells using 3-cyano-7-ethoxycoumarin (CEC) as a substrate. CYP1A2 gene expression is driven by the GAPDH promoter (GAPDHp). The results show that a CPR (i.e., yRD) is essential for CYP450 activity. (C). Determination of CYP1A2 enzymatic activity in yRD⁺:: PGK1-1A2 and yRD⁻:: PGK1-1A2 yeast cells using 3-cyano-7-ethoxycoumarin (CEC) as a substrate. CYP1A2 gene expression is driven by the PGK1 promoter (PGK1p). The results show that a CPR (i.e., yRD) is essential for CYP450 activity.