Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 21;20(14):4050. doi: 10.3390/s20144050

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

(A) Measurement of CYP1A1 activities in yeast strains, yRD⁺ (+CPR), yRD⁻:: Bax (–CPR), and yRD⁻ (–CPR), using 7-ethoxyresorufin (7-ER) as a substrate. CYP1A1 expression, in all three strains, is driven by the ethanol-inducible ADH2 promoter, whereas Bax expression is driven by the GAL1 promoter which is de-repressed in ethanol. The bar charts show that very low levels of Bax expression can indeed substitute for the absence of CPR (i.e., yeast CPR, yRD), which is essential for CYP activity. A between-group ANOVA test identified significant differences (p < 0.05) between strains, in all four comparative analyses performed. (B) Measurement of CYP1A2 activities in yeast strains, yRD⁺ (+CPR), yRD⁻:: Bax (–CPR) and yRD⁻ (–CPR), using 3-cyano-7-ethoxycoumarin (CEC) as a substrate. CYP1A2 expression, in all three strains, is driven by the ethanol-inducible ADH2 promoter, whereas Bax expression is driven by the GAL1 promoter which is de-repressed in ethanol. The bar charts show that very low levels of Bax expression can substitute for the absence of CPR (i.e., yeast CPR, yRD), which is essential for CYP activity. A between-group ANOVA test identified significant differences (p < 0.05) between strains, in all four comparative analyses performed. (C) Measurement of CYP1B1 activities in yeast strains, yRD+ (+CPR), yRD-:: Bax (–CPR) and yRD- (–CPR), using 7-ethoxyresorufin (7-ER) as a substrate. CYP1B1 expression, in all three strains, is driven by the ethanol-inducible ADH2 promoter, whereas Bax expression is driven by the GAL1 promoter which is de-repressed in ethanol. The Bar Charts show that very low levels of Bax expression can indeed substitute for the absence of CPR (i.e., yeast CPR, yRD), which is essential for CYP activity. A between-group ANOVA test identified significant differences (p < 0.05) between strains, in all four comparative analyses performed.