Figure 2.

General schematic of the new approach to probe–target hybridization for DRA. A sample containing dsDNA targets is supplemented with a specific biotinylated probe and subjected to DNA denaturation at 95 °C followed by quick incubation on ice. The denatured probe and target mixture are supplemented with horseradish peroxidase (HRP) covalently attached to an oligonucleotide tag for hybridization to the probe. The mixture is added to the streptavidin (SA)-coated solid carrier for attachment and hybridization of the specific targets and tagged HRP to the probes. After washing to remove the unbound molecules, the specific REase is added, catalyzing enzymatic cleavage and HRP release. The free HRP is transferred to a detection cell.