Table 1.
Characteristics of Virology Quality Assurance (VQA) specimens used in this study.
| Specimen | Viral Load a | Subtype b | PR DRMs c | RT DRMs c | % Mixed Bases in SS Consensus d | Number of Amplification Failures |
|---|---|---|---|---|---|---|
| 24.1 | 7815 | B | None | T215C | 2.3% | 0 |
| 24.2 | 18,023 | F | K20R, M36I | None | 0.0% | 0 |
| 24.3 | 26,372 | C | M36I | M41L, V75T, V90I, V106M, V179D | 0.0% | 0 |
| 24.4 | 29,139 | C | M36I | M41L, K103N, M184V, T215Y | 0.1% | 1 |
| 24.5 | 6424 | B | L10I, L33F, M46L, I54V, A71I/T, V82A, L90M | M41L, E44D, A62V, D67N, L74V, L100I, K103N, H208Y, L210W, T215Y H221Y | 0.8% | 1 |
| 26.1 | 16,685 | C | M36I, T74S | D67N, K70R, V90I, M184V | 0.9% | 0 |
| 26.2 e | 4513 | B | L10I, L33F, M46L, I54V, A71I/T, V82A, L90M | M41L, E44D, A62V, D67N, L74V, L100I, K103N, H208Y, L210W, T215Y, H221Y | 1.1% | 1 |
| 26.3 | 18,213 | C | K20R, M36I | A62V, K65R, D67N, V75A/I/T, K101Q, K103N, V106M, E138A, M184V | 2.1% | 1 |
| 26.4 | 6506 | D | M36I | None | 1.1% | 2 |
| 26.5 | 3656 | B | none | V90I, K103N | 3.8% | 0 |
a RNA copies/mL. b determined based on protease (PR)- reverse transcriptase (RT) sequence and Stanford HIVdb. c Drug resistance-associated mutation (DRM) sites were defined as any position with a potential impact on the penalty score in the Stanford HIVdb algorithm (version 8.5). d percentage of nucleotides in the VQA Sanger consensus sequence that are mixed, including positions where consensus was not reached. e same donor virus as 24.5.